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Setaria XRF and Autoradiographic Imaging

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(Top) X-ray fluorescence (XRF) images showing concentrations of different metals along the length of a Setaria grass root. (Bottom) Autoradiographic images of three positron emission tomography (PET) tracers in three different Setaria grass roots. In both image sets, a color scale shows lower concentrations as blue and higher concentrations as red. All roots were exposed to Azospirillum (Hm053 hyper nitrogen fixing) bacteria. These results show how the metal concentrations, using XRF imaging, can be correlated with the uptake of PET radiotracers in plant root systems, illuminating the mechanism by which the association between these bacteria and the plant root system improves the plant’s nitrogen uptake.

Credit or Source: Brookhaven National Laboratory


US DOE. 2014. New In Situ Imaging and Measurement Technologies for Biological Systems, US Department of Energy Office of Science. (PDF)

Prepared by the Biological and Environmental Research Information System, Oak Ridge National Laboratory, genomicscience.energy.gov/ and genomics.energy.gov/.