U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights

Measuring Chemical Changes Inside Living Cells
Published: March 01, 2011
Posted: April 08, 2011

Understanding how microbes adapt to changing chemical environments is a critical aspect of using them to solve DOE challenges. With synchrotron radiation-based Fourier transform infrared microscopy at the Advanced Light Source, researchers tracked the chemistry of living Desulfovibrio vulgaris cells in real time. The ability to make these dynamic measurements continuously inside selected living cells dramatically increases the usefulness and reliability of information traditionally derived from cells that have been killed and broken apart.

Reference: Holman, H.-Y., et al. 2009. “Real-Time Molecular Monitoring of Chemical Environment in Obligate Anaerobes During Oxygen Adaptive Response,” Proceedings of the National Academy of Sciences (USA) 106, 12599–604.

Topic Areas:

  • Research Area: Microbes and Communities
  • Research Area: Structural Biology, Biomolecular Characterization and Imaging
  • Research Area: Structural Biology Infrastructure

Division: SC-23.2 Biological Systems Science Division, BER


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