U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights

Addition of Bio-Magic Angle Spinning (MAS) Probe to 900-Megahertz Nuclear Magnetic Resonance (NMR) Spectrometer at the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL) Allows EMSL Users to Begin Studying the Structure of Proteins in the Solid State
Published: September 18, 2006
Posted: September 29, 2006

New EMSL capability advances the ability of the scientific community to determine the structures of membrane-bound proteins. One of the marquee capabilities at the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility located in Richland, Washington, is the 900 megahertz (MHz) nuclear magnetic resonance (NMR) spectrometer. A primary component of an NMR is the sample probe, which holds the sample, sends radiofrequency energy into the sample, and detects the signal emanating from the sample. A unique, Varian bio-magic angle spinning (MAS) probe recently has been coupled to the 900-MHz NMR at the EMSL and has helped EMSL users from the University of Illinois and PNNL begin to determine the structure of a small protein in the solid state. Chad Rienstra from the University of Illinois and Andrew Lipton from PNNL used the bio-MAS probe in EMSL's 900 MHz NMR to obtain high resolution and spectacular spectra of microcrystalline GB1, a streptococcal protein. These spectra will help to address ambiguities encountered by Dr. Rienstra in similar experiments using a 750-MHz NMR system. Although a few lower-field, bio-MAS probe/NMR systems exist, the new bio-MAS probe/900-MHz NMR system at EMSL is a unique, one-of-a-kind system.

Contact: Paul Bayer, SC-23.4, (301) 903-5324
Topic Areas:

  • Research Area: Subsurface Biogeochemical Research
  • Research Area: DOE Environmental Molecular Sciences Laboratory (EMSL)
  • Research Area: Structural Biology, Biomolecular Characterization and Imaging
  • Research Area: Research Technologies and Methodologies

Division: SC-23.1 Climate and Environmental Sciences Division, BER
      (formerly SC-23.4 Environmental Remediation Sciences Division, OBER)


BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

May 10, 2019
Quantifying Decision Uncertainty in Water Management via a Coupled Agent-Based Model
Considering risk perception can improve the representation of human decision-making processes in age [more...]

May 09, 2019
Projecting Global Urban Area Growth Through 2100 Based on Historical Time Series Data and Future Scenarios
Study provides country-specific urban area growth models and the first dataset on country-level urba [more...]

May 05, 2019
Calibrating Building Energy Demand Models to Refine Long-Term Energy Planning
A new, flexible calibration approach improved model accuracy in capturing year-to-year changes in bu [more...]

May 03, 2019
Calibration and Uncertainty Analysis of Demeter for Better Downscaling of Global Land Use and Land Cover Projections
Researchers improved the Demeter model’s performance by calibrating key parameters and establi [more...]

Apr 22, 2019
Representation of U.S. Warm Temperature Extremes in Global Climate Model Ensembles
Representation of warm temperature events varies considerably among global climate models, which has [more...]

List all highlights (possible long download time)