BER launches Environmental System Science Program. Visit our new website under construction!

U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


Comparative Microbial Genomic Comparisons on Single DNA Molecules
Published: November 15, 2004
Posted: December 02, 2004

Optical mapping is a technology developed by David Schwartz (University of Wisconsin) to directly image a "stretched-out" molecule of genomic DNA using the unique locations of restriction enzyme cut sites as orientation marks along the length of the DNA. Schwartz and collaborators have now used this powerful technology to directly compare single genomic DNA molecules from a series of different bacteria in order to identify and annotate DNA alterations between bacterial strains represented by several species. These results, published in the November issue of Journal of Bacteriology (v. 186 (22), pp. 7773-7782, 2004), suggest that genomic rearrangements and chromosomal breakpoints can be readily identified and annotated against a prototypic sequenced strain by using the tools of optical mapping. This will contribute to analysis of microbial genomes by comparative genomics which uses information derived from previously sequenced microbial species. To gain further insights, new sequencing efforts are now dealing with the variety of strains or isolates that gives a species definition and range; however, this number vastly outstrips our ability to sequence them.

Contact: Dan Drell, SC-72, (301) 903-4742
Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Microbes and Communities
  • Research Area: Research Technologies and Methodologies

Division: SC-33.2 Biological Systems Science Division, BER
      (formerly SC-72 Life Sciences Division, OBER)

 

BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

Mar 23, 2021
Molecular Connections from Plants to Fungi to Ants
Lipids transfer energy and serve as an inter-kingdom communication tool in leaf-cutter ants&rsqu [more...]

Mar 19, 2021
Microbes Use Ancient Metabolism to Cycle Phosphorus
Microbial cycling of phosphorus through reduction-oxidation reactions is older and more widespre [more...]

Feb 22, 2021
Warming Soil Means Stronger Microbe Networks
Soil warming leads to more complex, larger, and more connected networks of microbes in those soi [more...]

Jan 27, 2021
Labeling the Thale Cress Metabolites
New data pipeline identifies metabolites following heavy isotope labeling.

Analysis [more...]

Aug 31, 2020
Novel Bacterial Clade Reveals Origin of Form I Rubisco
Objectives

  • All plant biomass is sourced from the carbon-fixing enzyme Rub [more...]

List all highlights (possible long download time)