Enabled by JGI’s Emerging Technologies Opportunity Program, researchers at the Max Planck Institute (MPI) for Marine Microbiology developed, tested, and deployed a pipeline to first target cells from communities of uncultivated microbes, and then efficiently retrieve and characterize their genomes.
Specific bacterial cells are tagged with multiple fluorescent signals, collected through fluorescence activated cell sorting (FACS) and then enriched. These enriched populations are then sequenced and analyzed.
Grieb, A. et al. “A pipeline for targeted metagenomics of environmental bacteria.” Microbiome 8(1), 21 (2020). [DOI:10.1186/s40168-020-0790-7]
SC-33.2 Biological Systems Science Division, BER
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