Develop an in vitro approach to substantially accelerate the design and optimization of biosynthetic pathways to introduce into engineered cells.
The iPROBE cell-free framework was developed in which cell lysates are enriched with selected enzymes by overexpression or in vitro translation. Lysates are mixed to assemble multiple enzymatic pathway combinations. Productivity is then quantified and ranked to select the best pathways to implement in vivo.
Karim, A.S. et al, “In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design.” Nature Chemical Biology 16, 912–919 (2020). [DOI:10.1038/s41589-020-0559-0]
SC-33.2 Biological Systems Science Division, BER
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