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Identification of Critical Mutation in a Novel Regulator of Lignin Synthesis in Poplar
Published: January 03, 2020
Posted: August 05, 2021

Objective

Identify functional SNPs in Populus trichocarpa PtrEPSP-TF responsible for a novel transcriptional repressor regulating phenylpropanoid (PP) biosynthesis.

Approach

  • Used GWAS studies to predict high-impact SNPs in PtrEPSP-TF.
  • Characterized transgenic lines heterogeneously expressing PtrEPSP-TF and performed phenotypic and transcriptomic analyses to discover downstream regulators in the PP pathway.

Results/Impacts

  • Defined SNPs at the 142nd amino acid of PtrEPSP-TF as the primary determinator of function.
  • Uncovered 5 new TF negatively regulating PP biosynthesis.
  • Provides molecular targets for precision genome-editing to regulate PP biosynthesis to improve biomass feedstocks characteristics.

Xie, M. et al. “Identification of functional single nucleotide polymorphism of Populus trichocarpa PtrEPSP-TF and determination of its transcriptional effect.” Plant Direct 4(1), 1–13 (2020). [DOI:10.1002/pld3.178]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: DOE Bioenergy Research Centers (BRC)

Division: SC-33.2 Biological Systems Science Division, BER

 

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