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Agrobacterium-Mediated Transient Expression in Sorghum Leaves for Accelerating Functional Genomics and Genome Editing Studie
Published: February 27, 2020
Posted: August 05, 2021

Objective

Develop a transient gene expression system in sorghum to efficiently screen promoter-gene constructs.

Approach

  • Develop an Agrobacterium-mediated transient gene expression assay with intact sorghum leaves using green fluorescent protein as marker.
  • Test genome editing capability using sgRNA to GFP protein in sorghum.

Results/Impacts

  • In planta Agrobacterium-mediated transient expression of transgenes is achievable in sorghum leaves
  • High reproducibility, simplicity, rapidity, and feasibility to transform large constructs, which can directly be used for stable transformation.
  • This method can be used for subcellular localization studies and physiological assays.

Sharma, R. et al.Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies.” BMC Research Notes 13, 116 (2020). [DOI:10.1186/s13104-020-04968-9]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: DOE Bioenergy Research Centers (BRC)

Division: SC-33.2 Biological Systems Science Division, BER

 

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