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Methyl Ketone Production by Pseudomonas putida Is Enhanced by Plant-Derived Amino Acids
Published: April 14, 2019
Posted: August 03, 2021

Objective

Methyl ketones are a promising diesel blendstock and have been produced by multiple microbial platforms. Test use of plant-derived aromatics and amino acids to improve the efficiency of overall biomass conversion to methyl ketones.

Approach

  • Engineered Pseudomonas putida to produce methyl ketones by introducing a truncated beta-oxidation pathway.
  • Tested the strain on pure substrates and plant hydrolysates containing significant amounts of plant-derived aromatics.

Results/Impacts

  • Engineered P. putida produced methyl ketones from glucose and lignin-related aromatics.
  • Methyl ketone production by P. putida increased with plant hydrolysates as substrates in comparison to sugar controls.
  • Metabolomics and proteomics demonstrated that the increased production was due to plant-derived amino acids.
  • Inclusion of a defined amino acid mixture increased methyl ketone production 9-fold relative to a sugar-only control.

Dong et al. “Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids,” Biotechnology and Bioengineering 116(8), 1909–1922 (2019). [DOI:10.1002/bit.26995]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Microbes and Communities
  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: DOE Bioenergy Research Centers (BRC)

Division: SC-33.2 Biological Systems Science Division, BER

 

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