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One Poplar 5-Enolpyruvylshikimate 3-Phosphate (EPSP) Synthase Moonlight as Transcriptional Repressor to Regulate Lignin Biosynthesis
Published: June 11, 2018
Posted: August 02, 2021

Objective

Understand how lignin biosynthesis is regulated in poplar.

Approach

  • Used GWAS to define the linkage of PtrEPSP-TF and lignin biosynthesis.
  • Validated linkage by assessing PtrEPSP-TF overexpressing poplars for lignin deposition, secondary metabolism, and gene expression levels.
  • Investigated underlying molecular mechanism by protoplast-based assays.

Result/Impacts

  • EPSP synthase shown to co-function as a transcriptional repressor.
  • PtrEPSP-TF overexpressing poplars had increased lignin deposition, accumulation of lignin-related metabolites, and expression of lignin-related genes.
  • PtrEPSP-TF directly represses the expression of PtrhAT. PtrhAT then directly represses the expression of PtrMYB021. Consequently, PtrEPSP-TF positively regulates PtrMYB021 expression and lignin biosynthesis in poplar.

Xie, M. et al. “A 5-enolpyruvylshikimate 3-phosphate synthase functions as a transcriptional repressor in Populus.” The Plant Cell 30(7), 1645–1660 (2018). [DOI:10.1105/tpc.18.00168]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: DOE Bioenergy Research Centers (BRC)

Division: SC-33.2 Biological Systems Science Division, BER

 

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