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Towards the Synthetic Design of Camelina Oil Enriched in Tailored Acetyl-Triacylglycerols with Medium-Chain Fatty Acids
Published: July 06, 2018
Posted: August 02, 2021

Objective

To produce 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) with medium-chain fatty acids (MCFAs) in the oilseed crop Camelina sativa by metabolic engineering.

Approach

  • Different transgenic camelina lines that had been genetically modified to produce MCFAs through the expression of MCFA-specific thioesterases and acyltransferases were retransformed with the Euonymus alatus gene for diacylglycerol acetyltransferase (EaDAcT) that synthesizes acetyl-TAGs.
  • Concomitant RNAi suppression of acyl-CoA: diacylglycerol acyltransferase was designed to increase the levels of acetyl-TAGs.

Result/Impacts

  • Successfully generated metabolically engineered camelina that produce acetyl-TAG molecules containing medium-chain fatty acids.
  • These tailored lipids with a designed structure are potentially useful for production of biofuels and bioproducts in crops such as sorghum.

Bansal S, et al. “Towards the synthetic design of camelina oil enriched in tailored acetyl-triacylglycerols with medium-chain fatty acids.” Journal of Experimental Botany 69(18), 4395–4402 (2018). [DOI:10.1093/jxb/ery225]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: DOE Bioenergy Research Centers (BRC)
  • Research Area: Biosystems Design

Division: SC-33.2 Biological Systems Science Division, BER

 

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