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BER Research Highlights


Magic Pools: Parallel Assessment of Transposon Delivery Vectors in Bacteria
Published: January 16, 2018
Posted: August 02, 2021

Objective

Streamlined approaches for testing hundreds to thousands of genetic systems in parallel against a target bacterium.

Approach

  • Avoids the labor and cost associated with trial-and-error based approaches.
  • Opens door for large-scale genetic studies in diverse bacteria.

Results/Impact

  • Uses a “parts-based” synthetic biology cloning strategy.
  • Uses random DNA barcode sequences to track the efficacy of many transposon vectors in parallel.
  • Successfully adapted the strategy to 5 different genera of bacteria, including 3 from the phylum Bacteroidetes.

Liu, H. et al. “Magic pools: Parallel assessment of transposon delivery vectors in bacteria.” mSystems 3(1) e00143-17 (2017). [DOI:10.1128/mSystems.00143-17]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Microbes and Communities

Division: SC-33.2 Biological Systems Science Division, BER

 

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