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Novel Gene Targets Discovered for Improving Heterologous Enzyme Production in Aspergillus Niger
Published: January 06, 2018
Posted: August 02, 2021

Objective

Enhance the production of secreted heterologous proteins in fungal host A. niger.

Approach

  • Developed a forward genetics screen coupled with whole-genome resequencing to identify specific lesions responsible for a protein hyper-production phenotype in A. niger.
  • Use bioinformatics to identify loci associated with heterologous enzyme hyper-production.

Results/Impact

  • Strategy successfully identified novel targets, including a low-affinity glucose transporter, MstC, whose deletion significantly improved secretion of recombinant proteins driven by a glucoamylase promoter. Deletion of mstC loci improved by 4-fold heterologous enzyme production driven by a glucoamylase promoter.
  • Mutations found in the Ypt/Rab GTPase-activating protein of both tested strains might further increase production of heterologous beta-glucosidase if introduced into other heterologous strains.
Reilly, M.C. et al. “Forward genetics screen coupled with whole-genome resequencing identifies novel gene targets for improving heterologous enzyme production in Aspergillus niger.” Applied Microbiology and Biotechnology 102(4), 1797–1807 (2018). [DOI:10.1007/s00253-017-8717-3]

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Microbes and Communities

Division: SC-33.2 Biological Systems Science Division, BER

 

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