Scarce compound is key for microbial growth and may help shape microbial communities.
Vitamin B12 regulates the production of deoxyribonucleic acid (DNA) and many proteins required for normal cellular function. A recent study revealed that this compound plays an even greater role in cellular metabolism and growth than previously thought, and may even coordinate the behavior of complex microbial communities.
The findings suggest that vitamin B12 helps shape microbial communities, which affect wide-ranging processes including energy and food production, the environment, and human health.
Vitamin B12 is used by all domains of life to control the production of DNA and a variety of proteins that support cellular function, but this vitamin is only produced by certain bacterial and archaeal species. A recent study showed that this compound has an unexpectedly broad influence on metabolic processes important for synthesis of DNA, ribonucleic acid (RNA), and proteins. To explore vitamin B12’s role in a variety of cellular processes, researchers from Pacific Northwest National Laboratory, Sanford-Burnham-Prebys Medical Discovery Institute, and Polytech Nice-Sophia set out to identify which proteins bind to vitamin B12. To do so, they first developed a chemical probe that mimics vitamin B12 and then directly applied the probe to live Halomonas bacterial cells. The researchers next analyzed the probe-labeled proteins using an Orbitrap mass spectrometer at the Environmental Molecular Sciences Laboratory, a Department of Energy Office of Science user facility. They found that the vitamin B12-mimicking probe interacted with 41 different proteins, including enzymes involved in the synthesis and metabolism of another B vitamin called folate, an amino acid called methionine, and a compound called ubiquinone. These metabolic processes, in turn, increase the production of DNA, RNA, and proteins. The findings reveal vitamin B12 plays a more pivotal role in cellular growth and metabolism than previously thought. As a result, this scarce compound may facilitate the coordination of cell behavior in complex microbial communities, shaping their structure, stability, and overall function.
BER PM Contact
Paul Bayer, SC-23.1, 301-903-5324
Lee Ann McCue
Environmental Molecular Sciences Laboratory
This work was supported by the U.S. Department of Energy (DOE), Office of Science, Office of Biological and Environmental Research, including support of the Environmental Molecular Sciences Laboratory, a DOE Office of Science user facility; Genome Science Program Foundational Scientific Focus Area at Pacific Northwest National Laboratory; Russian Foundation for Basic Research; and Russian Academy of Sciences.
M. Romine, D. Rodionov, Y. Maezato, L. Anderson, P. Nandhikonda, I. Rodionova, A. Carre, X. Li, C. Xu, T. Clauss, Y.-M. Kim, T. Metz, and A. T. Wright, “Elucidation of roles for vitamin B12 in regulation of folate, ubiquinone, and methionine metabolism.” Proceedings of the National Academy of Sciences (USA) 114(7), E1205-E1214 (2017). DOI: 10.1073/pnas.1612360114. (Reference link)
PNNL News Release
SC-33.1 Earth and Environmental Sciences Division, BER
BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER
Mar 23, 2021
Molecular Connections from Plants to Fungi to Ants
Lipids transfer energy and serve as an inter-kingdom communication tool in leaf-cutter ants&rsqu [more...]
Mar 19, 2021
Microbes Use Ancient Metabolism to Cycle Phosphorus
Microbial cycling of phosphorus through reduction-oxidation reactions is older and more widespre [more...]
Feb 22, 2021
Warming Soil Means Stronger Microbe Networks
Soil warming leads to more complex, larger, and more connected networks of microbes in those soi [more...]
Jan 27, 2021
Labeling the Thale Cress Metabolites
New data pipeline identifies metabolites following heavy isotope labeling.
Aug 31, 2020
Novel Bacterial Clade Reveals Origin of Form I Rubisco
List all highlights (possible long download time)