U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


A Novel Lipid Pathway Makes Massive Quantity of Surface Wax on Bayberry Fruit
Published: January 07, 2016
Posted: May 02, 2016

Pathway gives metabolic engineers new tools for producing high-value lipids.

The Science
Bayberry fruits produce the highest amount of surface wax known in nature. Recent biochemical and gene expression data have revealed a novel biosynthesis pathway for the waxy layer that is more closely related to cutin biosynthesis than conventional triacylglyceride biosynthesis.

The Impact
The discovery of how the Bayberry fruit produces massive amounts of unique surface wax aids in understanding the plant’s mechanism for producing and secreting nonmembrane glycerolipids and suggests ways to engineer pathways for high-value waxy lipid production in biomass crops.

Summary
Scientists from the Department of Energy’s (DOE) Great Lakes Bioenergy Research Center (GLBRC) studied how Bayberry fruits accumulate massive quantities of a unique surface wax with a structure similar to triacylglycerol seed oils. Research on plants that produce such large amounts of surface lipids is providing insights into the molecular features and biochemical pathways for plant lipid secretion and thus may help in developing strategies to engineer lipid production in non-seed tissues. The GLBRC scientists examined changes in fruit anatomy and details of the chemical structures secreted by Bayberry fruits, and quantified the accumulation of wax through fruit development. Biochemical pathway analysis by [14C]-labeling and transcript analysis by RNA-seq revealed features of Bayberry wax accumulation that are distinctly different from conventional triacylglycerol production. Together, these results indicate that the extracellular glycerolipids in Bayberry wax are synthesized by a novel pathway that differs from previously defined triacylglycerol biosynthesis pathways. An increased understanding of this process may prove useful in engineering plants for secretion of high-energy and high-value lipids, particularly those that have toxic or negative consequences when accumulated inside cells.

Contacts
(BER PM)

N. Kent Peters, SC-23.2, kent.peters@science.doe.gov, 301-903-5549

(PI Contact)
John B. Ohlrogge
Michigan State University
ohlrogge@msu.edu

Funding
This work was funded by GLBRC (DOE, Office of Science, Office of Biological and Environmental Research DE-FC02-07ER64494) and a National Science and Engineering Research Council of Canada postgraduate fellowship (PGS-D3).

Publications
Simpson, J. P., and J. B. Ohlrogge. 2016. “A Novel Pathway for Triacylglycerol Biosynthesis Is Responsible for the Accumulation of Massive Quantities of Glycerolipids in the Surface Wax of Bayberry (Myrica pensylvanica) Fruit, The Plant Cell 28(1), 248–64. DOI: 10.1105/tpc.15.00900. (Reference link)

Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: DOE Bioenergy Research Centers (BRC)
  • Research Area: Biosystems Design

Division: SC-23.2 Biological Systems Science Division, BER

 

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