U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights

Optimizing Microbial Bioproduction of Fuels
Published: December 14, 2015
Posted: April 27, 2016

Identifying factors that contribute to cell-to-cell variability in lipid production.

The Science
Microbial strains engineered to produce a large amount of lipids hold tremendous promise for the production of biofuels and chemicals. A recent study shed light on underlying causes of microbial cell-to-cell variability in lipid production.

The Impact
The findings revealed that conditions within cells and in the surrounding environment interact to contribute to variability in lipid production. The new insights could lead to strategies that optimize the use of engineered microbial strains for the production of important biofuels and chemicals.

The microbial production of biofuels and chemicals often does not reach the theoretical maximum yield, even for engineered strains, thereby limiting the reliability of large-scale bioprocessing. To understand the limitations, scientists have started to investigate the reasons for phenotypic diversity of cells within a culture. A team of scientists from the University of Idaho, Environmental Molecular Sciences Laboratory (EMSL), and Massachusetts Institute of Technology used advanced microfluidics combined with Epifluorescent and Raman microscopy at EMSL to study differences in the ability of individual cells of low-yield and high-yield strains of the fungus Yarrowia lipolytica to produce lipids. The researchers found lipid production fluctuated sporadically with time in both strains. The researchers labeled this newly discovered phenomenon “bioprocessing noise.” Furthermore, the high-yield fungal strain showed reduced bioprocessing noise in lipid production than the low-yield fungal strain. This finding indicates differences in the activity of key metabolic genes that contribute to bioprocessing noise and thus cellular diversity in lipid production. Moreover, this variability was amplified by environmental factors such as chemical gradients of nutrients or waste products surrounding cells. Taken together, these findings show extracellular and intracellular fluctuations interact to place an upper limit on the reliability of lipid production and total yield of lipids. This research could pave the way for new strategies to improve the reliability and efficiency of using engineered microbial strains for the production of lipids that could then be converted to valuable biofuels or chemicals.

BER PM Contact
Paul Bayer, SC-23.1, 301-903-5324

PI Contacts
Andreas Vasdekis
University of Idaho

Gregory Stephanopoulos
Massachusetts Institute of Technology

This work was supported by the U.S. Department of Energy’s Office of Science, Office of Biological and Environmental Research, including support of EMSL, a DOE Office of Science user facility; National Institute of General Medical Sciences of the National Institutes of Health; and a Linus Pauling Fellowship from Pacific Northwest National Laboratory.

Vasdekis, A. E., A. M. Silverman, and G. Stephanopoulos. 2015. “Origins of Cell-to-Cell Bioprocessing Diversity and Implications of the Extracellular Environment Revealed at the Single-Cell Level,” Nature Scientific Reports 5(17689), DOI: 10.1038/srep17689. (Reference link)

Related Links
EMSL article

Topic Areas:

  • Research Area: DOE Environmental Molecular Sciences Laboratory (EMSL)
  • Research Area: Microbes and Communities
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: Biosystems Design

Division: SC-23.1 Climate and Environmental Sciences Division, BER


BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

Aug 24, 2019
New Approach for Studying How Microbes Influence Their Environment
A diverse group of scientists suggests a common framework and targeting of known microbial processes [more...]

Aug 08, 2019
Nutrient-Hungry Peatland Microbes Reduce Carbon Loss Under Warmer Conditions
Enzyme production in peatlands reduces carbon lost to respiration under future high temperatures. [more...]

Aug 05, 2019
Amazon Forest Response to CO2 Fertilization Dependent on Plant Phosphorus Acquisition
AmazonFACE Model Intercomparison. The Science Plant growth is dependent on the availabi [more...]

Jul 29, 2019
A Slippery Slope: Soil Carbon Destabilization
Carbon gain or loss depends on the balance between competing biological, chemical, and physical reac [more...]

Jul 15, 2019
Field Evaluation of Gas Analyzers for Measuring Ecosystem Fluxes
How gas analyzer type and correction method impact measured fluxes. The Science A side- [more...]

List all highlights (possible long download time)