Through metagenomics, researchers sequenced 86 organisms from the phylum Chloroflexi, representing 15 distinct lineages, to discover the secrets of microbial life within terrestrial aquifer sediment deposits.
These Chloroflexi microbes were found to have metabolic processes involved in plant biomass degradation, which could be useful for biofuels production, as well as a better understanding of the subsurface nitrogen and carbon cycles. Microorganisms in aquifer sediments are responsible for subterranean carbon turnover and the degradation of organic contaminants. Consequently, these microorganisms can heavily impact the quality of underground drinking water. In earlier studies, it was determined that Chloroflexi represent a significant amount of the microbial population in sediments. However, these microbes are poorly understood, as only six of about 30 Chloroflexi classes have been sequenced. For this reason, a team of researchers including scientists from the Department of Energy’s Joint Genome Institute (DOE JGI) conducted a study on the microbial composition of these aquifer sediments to gain a broader knowledge of the metabolic characteristics of Chloroflexi microbes.
The researchers were able to reconstruct three near-complete Chloroflexi genomes from the metagenomic data collected at the Integrated Field-Scale Subsurface Research Challenge Site in Colorado as part of a DOE JGI Community Sequencing Program project led by Jill Banfield of the University of California, Berkeley. Metabolic analyses revealed that Chloroflexi can break down plant mass, influence subsurface carbon and nitrogen cycles, and adapt to changing oxygen levels. These traits, the researchers noted, were likely to apply to Chloroflexi in other sediment environments, making these microbes good candidates for mining useful enzymes and pathways for DOE missions of bioenergy and carbon processing as well as for biodegradation.
Reference: Hug, L. A., et al. 2013. “Community Genomic Analyses Constrain the Distribution of Metabolic Traits Across the Chloroflexi Phylum and Indicate Roles in Sediment Carbon Cycling,” Microbiome 1(22), DOI:10.1186/2049-2618-1-22. (Reference link)
Contact: Dan Drell, SC-23.2, (301) 903-4742
SC-23.2 Biological Systems Science Division, BER
BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER
May 10, 2019
Quantifying Decision Uncertainty in Water Management via a Coupled Agent-Based Model
Considering risk perception can improve the representation of human decision-making processes in age [more...]
May 09, 2019
Projecting Global Urban Area Growth Through 2100 Based on Historical Time Series Data and Future Scenarios
Study provides country-specific urban area growth models and the first dataset on country-level urba [more...]
May 05, 2019
Calibrating Building Energy Demand Models to Refine Long-Term Energy Planning
A new, flexible calibration approach improved model accuracy in capturing year-to-year changes in bu [more...]
May 03, 2019
Calibration and Uncertainty Analysis of Demeter for Better Downscaling of Global Land Use and Land Cover Projections
Researchers improved the Demeter model’s performance by calibrating key parameters and establi [more...]
Apr 22, 2019
Representation of U.S. Warm Temperature Extremes in Global Climate Model Ensembles
Representation of warm temperature events varies considerably among global climate models, which has [more...]
List all highlights (possible long download time)