U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


Using Mass Spectrometry to Localize Lipid Metabolites in Camelina Seeds
Published: August 05, 2013
Posted: February 07, 2014

Camelina sativa is a nonfood oilseed crop that, because of relatively low production costs and potential for use in a number of industrial applications, shows promise as a bioenergy feedstock. Additionally, the relative ease with which the plant can be genetically modified offers potential for altering the seed oil composition through engineering of the lipid and fatty acid metabolic pathways. To do this, however, it is important to understand how these pathways are regulated in different seed tissues. With funding from the Department of Energy’s Office of Science Genomic Science Program, researchers from the University of North Texas used mass spectrometry imaging techniques to show that the distribution of various lipid-related metabolites and precursors are specific to certain distinct tissues within the seed embryo. This high-resolution metabolite mapping in Camelina seeds can be used to reveal new insights into tissue-based variation and illustrates the importance of considering spatial heterogeneity when designing metabolic engineering strategies for manipulating seed lipid composition. This work will facilitate more refined and accurate targeting when engineering plants for optimal seed oil composition.
 
Reference: Horn, P. J., J. E. Silva, D. Anderson, J. Fuchs, L. Borisjuk, T. J. Nazarenus, V. Shulaev, E. B. Cahoon, and K. D. Chapman. 2013. “Imaging Heterogeneity of Membrane and Storage Lipids in Transgenic Camelina Sativa Seeds with Altered Fatty Acid Profiles,” The Plant Journal 76(1), 138 €"50. DOI:10.1111/tpj.12278. (Reference link)

Contact: Cathy Ronning, SC-23.2, (301) 903-9549
Topic Areas:

  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: Biosystems Design
  • Research Area: Research Technologies and Methodologies

Division: SC-23.2 Biological Systems Science Division, BER

 

BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

Aug 24, 2019
New Approach for Studying How Microbes Influence Their Environment
A diverse group of scientists suggests a common framework and targeting of known microbial processes [more...]

Aug 08, 2019
Nutrient-Hungry Peatland Microbes Reduce Carbon Loss Under Warmer Conditions
Enzyme production in peatlands reduces carbon lost to respiration under future high temperatures. [more...]

Aug 05, 2019
Amazon Forest Response to CO2 Fertilization Dependent on Plant Phosphorus Acquisition
AmazonFACE Model Intercomparison. The Science Plant growth is dependent on the availabi [more...]

Jul 29, 2019
A Slippery Slope: Soil Carbon Destabilization
Carbon gain or loss depends on the balance between competing biological, chemical, and physical reac [more...]

Jul 15, 2019
Field Evaluation of Gas Analyzers for Measuring Ecosystem Fluxes
How gas analyzer type and correction method impact measured fluxes. The Science A side- [more...]

List all highlights (possible long download time)