BER launches Environmental System Science Program. Visit our new website under construction!

U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


First Quantification of Total Thiols on Bacteria and Natural Organic Matter in Environmental Samples
Published: November 09, 2013
Posted: February 07, 2014

Organic thiols react and form complexes with some toxic soft metals such as mercury in both biotic and abiotic systems. However, a clear understanding of these interactions is currently limited because quantifying thiols in environmental matrices is difficult due to their low abundance, susceptibility to oxidation, and measurement interference by non-thiol compounds in samples. A team of scientists from Oak Ridge National Laboratory has developed a fluorescence-labeling method to determine total thiols directly on gram-negative bacterial cells and natural organic matter (NOM) in environmental samples. The method is highly selective and can quantify thiols at submicromolar concentration levels. The direct quantification of organic thiols on NOM and bacterial cells is needed to enable a mechanistic understanding of soft metal and biota interactions, metal speciation, and bioavailability.

Reference: Rao, B., C. Simpson, H. Lin, L. Liang, and B. Gu. 2014. “Determination of Thiol Functional Groups on Bacteria and Natural Organic Matter in Environmental Systems,” Talanta 119, 240-47. (Reference link)

Contact: Paul E. Bayer, SC-23.1, (301) 903-5324
Topic Areas:

  • Research Area: Subsurface Biogeochemical Research
  • Research Area: Microbes and Communities

Division: SC-33.1 Earth and Environmental Sciences Division, BER

 

BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

Mar 23, 2021
Molecular Connections from Plants to Fungi to Ants
Lipids transfer energy and serve as an inter-kingdom communication tool in leaf-cutter ants&rsqu [more...]

Mar 19, 2021
Microbes Use Ancient Metabolism to Cycle Phosphorus
Microbial cycling of phosphorus through reduction-oxidation reactions is older and more widespre [more...]

Feb 22, 2021
Warming Soil Means Stronger Microbe Networks
Soil warming leads to more complex, larger, and more connected networks of microbes in those soi [more...]

Jan 27, 2021
Labeling the Thale Cress Metabolites
New data pipeline identifies metabolites following heavy isotope labeling.

Analysis [more...]

Aug 31, 2020
Novel Bacterial Clade Reveals Origin of Form I Rubisco
Objectives

  • All plant biomass is sourced from the carbon-fixing enzyme Rub [more...]

List all highlights (possible long download time)