U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights

Carbon-11 Azelaic Acid as a Signaling Molecule for Mechanistic Studies in Plants
Published: April 25, 2013
Posted: October 23, 2013

When a pathogen attacks a plant, the plant mounts an immune response that alerts the rest of the plant, a response called systemic acquired resistance (SAR). The chemical compound(s) responsible for inducing the immunity is a topic of intense interest for agriculture, including for bioenergy crops. For example, the application of a 9-carbon-atom-chain (C-9) dicarboxylic acid, azaleic acid, induces immunity, but the similar C-8 and C-10 diacids do not. One hypothesis is that the azaleic acid, but not the related acids, moves to distant parts of the plant. New radiochemistry imaging research at Brookhaven National Laboratory has developed a rapid method to label these three acids with Carbon-11 (11C, half-life of 20.4 min) for short-term (minutes to hours) tracking of their movement within the plant, and with Carbon-14 (14C, half-life of 5730 years) for long-term (hours to days) studies. When applied to a leaf, [11C]-azaleic acid shows substantial movement within an hour. When [14C]-azaleic acid is applied to the roots, it distributes throughout the whole plant within a day. These studies demonstrate that azaleic acid has the potential to be a mobile signaling molecule. The radioactive-carbon labeled diacids will have utility as scientific tools to unravel SAR mechanisms and other phenomena that impact production of robust bioenergy crops.

References: Yu, K., J. M. Soares, M. K. Mandal, C. Wang, B. Chanda, A. N. Gifford, J. S. Fowler, D. Navarre, A. Kachroo, and P. Kachroo. 2013. “A Feedback Regulatory Loop Between G3P and Lipid Transfer Proteins DIR1 and AZI1 Mediates Azelaic-Acid-Induced Systemic Immunity,” Cell Reports 3, 1266–78. DOI: 10.1016/j.celrep.2013.03.030. (Reference link)

Best, M., A. N. Gifford, S. W. Kim, B. Babst, M. Piel, F. Roesch, J. S. Fowler. 2012. “Rapid Radiosynthesis of [11C] and [14C]Azaleic, Suberic, and Sebacic Acids for in vivo Mechanistic Studies of Systemic Acquired Resistance in Plants,” Journal of Labelled Compounds and Radiopharmaceuticals 55, 39-43. DOI: 10.1002/jlcr.1951. (Reference link)

Contact: Prem Srivastava, SC-23.2, (301) 903-4071
Topic Areas:

  • Research Area: Plant Systems and Feedstocks, Plant-Microbe Interactions
  • Research Area: Sustainable Biofuels and Bioproducts
  • Legacy: Radiochemistry and Instrumentation

Division: SC-23.2 Biological Systems Science Division, BER


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