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Improving Cyanobacterial Synthesis of Alkanes
Published: February 01, 2013
Posted: March 26, 2013

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Image Credit: Lawrence Berkeley National Laboratory" />

Alkane Biosynthesis. Comparison of visible and infrared images shows localized production of alkanes adhering to a cyanobacterium’s outer cell surface (represented by rainbow-colored speckles; red = maximum). more...

Image Credit: Lawrence Berkeley National Laboratory

Cyanobacteria are important photoautotrophic organisms that can capture carbon dioxide and convert it into a suite of organic compounds such as high-density liquid fuels. Using synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy as a high-throughput imaging method, researchers tracked metabolic phenotypes of Synechocystis 6803, which was engineered for enhanced production of alkanes and free fatty acids. Multivariate SR-FTIR data analysis revealed biochemical shifts in the engineered cells. These results demonstrate the applicability of SR-FTIR spectromicroscopy for rapid metabolic screening and phenotyping of live individual cells. The research was conducted using resources at the Advanced Light Source at Lawrence Berkeley National Laboratory.

Reference: Hu, P., et al. 2013. “Metabolic Phenotyping of the Cyanobacterium Synechocystis 6803 Engineered for Production of Alkanes and Free Fatty Acids,” Applied Energy 102, 850–59. (Reference link)

Contact: Roland F. Hirsch, SC-23.2, (301) 903-9009
Topic Areas:

  • Research Area: Microbes and Communities
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: Structural Biology, Biomolecular Characterization and Imaging
  • Research Area: Structural Biology Infrastructure

Division: SC-33.2 Biological Systems Science Division, BER

 

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