U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


Improving Cyanobacterial Synthesis of Alkanes
Published: February 01, 2013
Posted: March 26, 2013

more...

Image Credit: Lawrence Berkeley National Laboratory" />

Alkane Biosynthesis. Comparison of visible and infrared images shows localized production of alkanes adhering to a cyanobacterium’s outer cell surface (represented by rainbow-colored speckles; red = maximum). more...

Image Credit: Lawrence Berkeley National Laboratory

Cyanobacteria are important photoautotrophic organisms that can capture carbon dioxide and convert it into a suite of organic compounds such as high-density liquid fuels. Using synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy as a high-throughput imaging method, researchers tracked metabolic phenotypes of Synechocystis 6803, which was engineered for enhanced production of alkanes and free fatty acids. Multivariate SR-FTIR data analysis revealed biochemical shifts in the engineered cells. These results demonstrate the applicability of SR-FTIR spectromicroscopy for rapid metabolic screening and phenotyping of live individual cells. The research was conducted using resources at the Advanced Light Source at Lawrence Berkeley National Laboratory.

Reference: Hu, P., et al. 2013. “Metabolic Phenotyping of the Cyanobacterium Synechocystis 6803 Engineered for Production of Alkanes and Free Fatty Acids,” Applied Energy 102, 850–59. (Reference link)

Contact: Roland F. Hirsch, SC-23.2, (301) 903-9009
Topic Areas:

  • Research Area: Microbes and Communities
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: Structural Biology, Biomolecular Characterization and Imaging
  • Research Area: Structural Biology Infrastructure

Division: SC-23.2 Biological Systems Science Division, BER

 

BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

Aug 24, 2019
New Approach for Studying How Microbes Influence Their Environment
A diverse group of scientists suggests a common framework and targeting of known microbial processes [more...]

Aug 08, 2019
Nutrient-Hungry Peatland Microbes Reduce Carbon Loss Under Warmer Conditions
Enzyme production in peatlands reduces carbon lost to respiration under future high temperatures. [more...]

Aug 05, 2019
Amazon Forest Response to CO2 Fertilization Dependent on Plant Phosphorus Acquisition
AmazonFACE Model Intercomparison. The Science Plant growth is dependent on the availabi [more...]

Jul 29, 2019
A Slippery Slope: Soil Carbon Destabilization
Carbon gain or loss depends on the balance between competing biological, chemical, and physical reac [more...]

Jul 15, 2019
Field Evaluation of Gas Analyzers for Measuring Ecosystem Fluxes
How gas analyzer type and correction method impact measured fluxes. The Science A side- [more...]

List all highlights (possible long download time)