U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


Defeating NDM-1
Published: January 30, 2013
Posted: March 26, 2013

more... Image Credit: Argonne National Laboratory" />
NDM-1 Structure. NDM-1 structure in complex with two cadmium ions and antibiotic. Unhydrolyzed ampicillin is depicted in the active site. more... Image Credit: Argonne National Laboratory

The New Delhi metallo-beta-lactamase (NDM-1) gene makes multiple pathogenic microorganisms resistant to all known beta-lactam antibiotics including carbapenems, which are considered as “last resort” antibiotics. Researchers used the Advanced Photon Source at Argonne National Laboratory to determine the structural basis for NDM-1’s promiscuous activity via a combination of crystallographic and biochemical studies and theoretical calculations that elucidated a pH-dependent set of pathways. Based on these findings, future active drugs can be predicted.

Reference: Kim, Y., et al. 2013. “NDM-1, the Ultimate Promiscuous Enzyme: Substrate Recognition and Catalytic Mechanism,” The FASEB Journal, DOI: 10.1096/fj.12-224014. (Reference link)

Contact: Roland F. Hirsch, SC-23.2, (301) 903-9009
Topic Areas:

  • Research Area: Microbes and Communities
  • Research Area: Structural Biology, Biomolecular Characterization and Imaging
  • Research Area: Structural Biology Infrastructure

Division: SC-23.2 Biological Systems Science Division, BER

 

BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

Aug 24, 2019
New Approach for Studying How Microbes Influence Their Environment
A diverse group of scientists suggests a common framework and targeting of known microbial processes [more...]

Aug 08, 2019
Nutrient-Hungry Peatland Microbes Reduce Carbon Loss Under Warmer Conditions
Enzyme production in peatlands reduces carbon lost to respiration under future high temperatures. [more...]

Aug 05, 2019
Amazon Forest Response to CO2 Fertilization Dependent on Plant Phosphorus Acquisition
AmazonFACE Model Intercomparison. The Science Plant growth is dependent on the availabi [more...]

Jul 29, 2019
A Slippery Slope: Soil Carbon Destabilization
Carbon gain or loss depends on the balance between competing biological, chemical, and physical reac [more...]

Jul 15, 2019
Field Evaluation of Gas Analyzers for Measuring Ecosystem Fluxes
How gas analyzer type and correction method impact measured fluxes. The Science A side- [more...]

List all highlights (possible long download time)