U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


Defeating NDM-1
Published: January 30, 2013
Posted: March 26, 2013

more... Image Credit: Argonne National Laboratory" />
NDM-1 Structure. NDM-1 structure in complex with two cadmium ions and antibiotic. Unhydrolyzed ampicillin is depicted in the active site. more... Image Credit: Argonne National Laboratory

The New Delhi metallo-beta-lactamase (NDM-1) gene makes multiple pathogenic microorganisms resistant to all known beta-lactam antibiotics including carbapenems, which are considered as “last resort” antibiotics. Researchers used the Advanced Photon Source at Argonne National Laboratory to determine the structural basis for NDM-1’s promiscuous activity via a combination of crystallographic and biochemical studies and theoretical calculations that elucidated a pH-dependent set of pathways. Based on these findings, future active drugs can be predicted.

Reference: Kim, Y., et al. 2013. “NDM-1, the Ultimate Promiscuous Enzyme: Substrate Recognition and Catalytic Mechanism,” The FASEB Journal, DOI: 10.1096/fj.12-224014. (Reference link)

Contact: Roland F. Hirsch, SC-23.2, (301) 903-9009
Topic Areas:

  • Research Area: Microbes and Communities
  • Research Area: Structural Biology, Biomolecular Characterization and Imaging
  • Research Area: Structural Biology Infrastructure

Division: SC-23.2 Biological Systems Science Division, BER

 

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