U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights


Identifying the Best Biofuel-Producing Microbes
Published: November 06, 2012
Posted: December 04, 2012

To use a microbe as a factory to make a desired product, a bacterial strain that already produces the compound is treated to generate many mutants, some of which may produce more of the product. From all these new variants, the challenge is to identify those microbes that make the largest amounts of the desired compound. This is particularly difficult when the target compound (e.g., a biofuel) does not confer any selective advantage to the microbe. To solve this problem, researchers at the U.S. Department of Energy’s (DOE) Lawrence Berkeley National Laboratory and DOE Joint BioEnergy Institute designed a “biosensor”—a genetic regulator that “senses” the presence of the desired product (e.g., butanol). The expression of a gene that confers an advantage to the microbe, such as resistance to the antibiotic tetracycline, is then induced by the presence of the biosensor. Butanol biosensor-containing Escherichia coli cells, for example, grow in the presence of the antibiotic only if the medium also contains butanol. Finally, plasmids capable of synthesizing various amounts of butanol were introduced into E. coli containing the butanol biosensor and growing in tetracycline-containing medium. High butanol-producing cells could readily be identified by their faster growth rates. This approach will facilitate the selection of microbial strains that produce large quantities of any small molecule, an important step toward the development of renewable biofuels.

Reference: Dietrich, J. A., D. L. Shis, A. Alikhani, and J. D. Keasling. 2012. “Transcription Factor-Based Screens and Synthetic Selections for Microbial Small-Molecule Biosynthesis,” ACS Synthetic Biology, DOI: 10.1021/sb300091d. (Reference link).

Contact: Pablo Rabinowicz, SC-23.2 (301) 903-0379
Topic Areas:

  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Microbes and Communities
  • Research Area: Sustainable Biofuels and Bioproducts
  • Research Area: DOE Bioenergy Research Centers (BRC)

Division: SC-23.2 Biological Systems Science Division, BER

 

BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

May 10, 2019
Quantifying Decision Uncertainty in Water Management via a Coupled Agent-Based Model
Considering risk perception can improve the representation of human decision-making processes in age [more...]

May 09, 2019
Projecting Global Urban Area Growth Through 2100 Based on Historical Time Series Data and Future Scenarios
Study provides country-specific urban area growth models and the first dataset on country-level urba [more...]

May 05, 2019
Calibrating Building Energy Demand Models to Refine Long-Term Energy Planning
A new, flexible calibration approach improved model accuracy in capturing year-to-year changes in bu [more...]

May 03, 2019
Calibration and Uncertainty Analysis of Demeter for Better Downscaling of Global Land Use and Land Cover Projections
Researchers improved the Demeter model’s performance by calibrating key parameters and establi [more...]

Apr 22, 2019
Representation of U.S. Warm Temperature Extremes in Global Climate Model Ensembles
Representation of warm temperature events varies considerably among global climate models, which has [more...]

List all highlights (possible long download time)