U.S. Department of Energy Office of Biological and Environmental Research

BER Research Highlights

Mapping Sensory Systems in Sulfate-Reducing Bacteria
Published: October 12, 2011
Posted: December 20, 2011

Sulfate-reducing bacteria (SRBs) play important roles in the decomposition of organic matter, cycling of nutrients, and transformation of heavy metals in subsurface environments. Sensing and responding to minute shifts in nutrient levels, potentially damaging or toxic conditions, and the presence of other microbes is critical to their lifestyle. Systems involving two components, paired sets of sensor and regulator proteins that control gene expression, are an important sense/response mechanism in bacteria, but it remains extremely difficult to establish relationships between the systems and larger networks of regulated genes. Researchers at Lawrence Berkeley National Laboratory have now completed the first-ever map of two-component regulatory systems for the model microbe SRB Desulfovibrio vulgaris using a cell-free approach based on direct binding of purified regulator proteins to genome fragments. Genes involved in nutrient acquisition, growth, stress response, and community assembly were mapped onto specific response regulators, providing a greatly enhanced understanding of how SRBs react to changing environmental conditions and mediate key processes in the subsurface.

Reference: Rajeev, L., E. G. Luning, P. S. Dehal, M. N. Price, A. P. Arkin, and A. Mukhopadhyay. 2011. “Systematic Mapping of Two Component Response Regulators to Gene Targets in a Model Sulfate Reducing Bacterium,” Genome Biology 12:R99. DOI:10.1186/gb-2011-12-10-r99. (Reference link)

Contact: Joseph Graber, SC-23.2, (301) 903-1239
Topic Areas:

  • Research Area: Subsurface Biogeochemical Research
  • Research Area: Genomic Analysis and Systems Biology
  • Research Area: Microbes and Communities

Division: SC-23.2 Biological Systems Science Division, BER


BER supports basic research and scientific user facilities to advance DOE missions in energy and environment. More about BER

Recent Highlights

May 10, 2019
Quantifying Decision Uncertainty in Water Management via a Coupled Agent-Based Model
Considering risk perception can improve the representation of human decision-making processes in age [more...]

May 09, 2019
Projecting Global Urban Area Growth Through 2100 Based on Historical Time Series Data and Future Scenarios
Study provides country-specific urban area growth models and the first dataset on country-level urba [more...]

May 05, 2019
Calibrating Building Energy Demand Models to Refine Long-Term Energy Planning
A new, flexible calibration approach improved model accuracy in capturing year-to-year changes in bu [more...]

May 03, 2019
Calibration and Uncertainty Analysis of Demeter for Better Downscaling of Global Land Use and Land Cover Projections
Researchers improved the Demeter model’s performance by calibrating key parameters and establi [more...]

Apr 22, 2019
Representation of U.S. Warm Temperature Extremes in Global Climate Model Ensembles
Representation of warm temperature events varies considerably among global climate models, which has [more...]

List all highlights (possible long download time)